- Abstract:
-
We compared and statistically evaluated the effectiveness of nine DNA extraction procedures by using frozen and dried samples of two silt loam soils and a silt loam wetland sediment with different organic matter contents. The effects of different chemical extractants (sodium dodecyl sulfate [SDS], chloroform, phenol, Chelex 100, and guanadinium isothiocyanate), different physical disruption methods (bead mill homogenization and freeze-thaw lysis), and lysozyme digestion were evaluated based o...
Expand abstract - Publication status:
- Published
- Journal:
- Applied and environmental microbiology
- Volume:
- 65
- Issue:
- 11
- Pages:
- 4715-4724
- Publication date:
- 1999-11-05
- EISSN:
-
1098-5336
- ISSN:
-
0099-2240
- URN:
-
uuid:74486aec-7230-4782-ad4d-ad6e73a375fa
- Source identifiers:
-
195580
- Local pid:
- pubs:195580
- Copyright date:
- 1999
Journal article
Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples.
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