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Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples.

Abstract:

We compared and statistically evaluated the effectiveness of nine DNA extraction procedures by using frozen and dried samples of two silt loam soils and a silt loam wetland sediment with different organic matter contents. The effects of different chemical extractants (sodium dodecyl sulfate [SDS], chloroform, phenol, Chelex 100, and guanadinium isothiocyanate), different physical disruption methods (bead mill homogenization and freeze-thaw lysis), and lysozyme digestion were evaluated based o...

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Publication status:
Published

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Authors


Miller, DN More by this author
Bryant, JE More by this author
Madsen, EL More by this author
Ghiorse, WC More by this author
Journal:
Applied and environmental microbiology
Volume:
65
Issue:
11
Pages:
4715-4724
Publication date:
1999-11-05
EISSN:
1098-5336
ISSN:
0099-2240
URN:
uuid:74486aec-7230-4782-ad4d-ad6e73a375fa
Source identifiers:
195580
Local pid:
pubs:195580

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