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The radical-SAM enzyme Viperin catalyzes reductive addition of a 5'-deoxyadenosyl radical to UDP-glucose in vitro.

Abstract:

Viperin, a radical-SAM enzyme conserved from fungi to humans, can restrict replication of many viruses. Neither the molecular mechanism underlying the antiviral activity of Viperin, nor its exact physiological function, is understood: most importantly, no radical-SAM activity has been discovered for Viperin. Here, using electron paramagnetic resonance (EPR) spectroscopy, mass spectrometry, and NMR spectroscopy, we show that UDP-glucose is a substrate of a fungal Viperin (58% pairwise identity...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted manuscript

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Publisher copy:
10.1002/1873-3468.12769

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Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
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Department:
Oxford, MSD, Biochemistry
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Department:
Merton College
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Department:
Oxford, MPLS, Chemistry, Organic Chemistry
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Department:
Oxford, MPLS, Chemistry, Inorganic Chemistry
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Author
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Professor Wilfred R. Hagen More from this funder
European Molecular Biology Organization More from this funder
Diabetes UK More from this funder
Royal Society More from this funder
Publisher:
Wiley Publisher's website
Journal:
FEBS Letters Journal website
Volume:
591
Issue:
16
Pages:
2394-2405
Publication date:
2017-07-28
Acceptance date:
2017-07-25
DOI:
EISSN:
1873-3468
ISSN:
0014-5793
Pubs id:
pubs:709866
URN:
uri:73e53115-d11f-4d63-b495-8daf66d694b7
UUID:
uuid:73e53115-d11f-4d63-b495-8daf66d694b7
Local pid:
pubs:709866
Language:
English
Keywords:

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