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Structure and kinetic characterization of human sperm-specific glyceraldehyde-3-phosphate dehydrogenase, GAPDS.

Abstract:

hGAPDS (human sperm-specific glyceraldehyde-3-phosphate dehydrogenase) is a glycolytic enzyme essential for the survival of spermatozoa, and constitutes a potential target for non-hormonal contraception. However, enzyme characterization of GAPDS has been hampered by the difficulty in producing soluble recombinant protein. In the present study, we have overexpressed in Escherichia coli a highly soluble form of hGAPDS truncated at the N-terminus (hGAPDSΔN), and crystallized the homotetrameric e...

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Journal:
The Biochemical journal
Volume:
435
Issue:
2
Pages:
401-9
Publication date:
2011-04-15
ISSN:
1470-8728
URN:
uuid:73d896bf-1147-4b2b-b372-d3e299e4436e
Local pid:
SGC:21269272
Language:
English

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