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Combining M-FISH and Quantum Dot technology for fast chromosomal assignment of transgenic insertions.

Abstract:

BACKGROUND: Physical mapping of transgenic insertions by Fluorescence in situ Hybridization (FISH) is a reliable and cost-effective technique. Chromosomal assignment is commonly achieved either by concurrent G-banding or by a multi-color FISH approach consisting of iteratively co-hybridizing the transgenic sequence of interest with one or more chromosome-specific probes at a time, until the location of the transgenic insertion is identified. RESULTS: Here we report a technical development fo...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1186/1472-6750-11-121

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Institution:
University of Oxford
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Author
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Institution:
University of Oxford
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Author
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Institution:
University of Oxford
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Author
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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Neuroscience, Biomedical Research Centre
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Author
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Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
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Author
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Funding agency for:
Yusuf, M
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Funding agency for:
Bauer, DL
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Funding agency for:
Lipinski, DM
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Funding agency for:
Maclaren, RE
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Publisher:
BioMed Central Ltd. Publisher's website
Journal:
BMC biotechnology Journal website
Volume:
11
Issue:
1
Pages:
121
Publication date:
2011-01-01
DOI:
EISSN:
1472-6750
ISSN:
1472-6750
URN:
uuid:7385013d-37c4-4dfe-99eb-1048cf3f05c9
Source identifiers:
221332
Local pid:
pubs:221332

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