Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart.

Journal article

Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological analysis, however, remains technically challenging, and the resulting quality is often unsatisfactory. For this report we have scrutinized each step involved in standard double-staining histology. We h...

Expand abstract

Authors: Bohl, S; Medway, D; Schulz-Menger, J; Schneider, J; Neubauer, S

• Publication status: Published
• Journal: American journal of physiology. Heart and circulatory physiology
• Volume: 297
• Issue: 6
• Pages: H2054-H2058
• Publication date: 2009-12-01
• DOI: 10.1152/ajpheart.00836.2009
• EISSN: 1522-1539
• ISSN: 0363-6135
• Language: English
• Keywords: Animals; Myocardial Reperfusion Injury; Severity of Illness Index; Myocardial Infarction; Mice; Indoles; Disease Models, Animal; Myocardium; Coloring Agents; Reproducibility of Results; Organometallic Compounds; Image Interpretation, Computer-Assisted; Staining and Labeling