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Journal article

Culture and differentiation of rat neural stem/progenitor cells in a three-dimensional collagen scaffold.

Abstract:

A stable and fast method for constructing a neural-like tissue from rat neural stem/progenitor cells (rNS/PCs) based on three-dimensional (3D) collagen gel is described. First step, the collagen-embedded rNS/PCs expanded with the medium consisting of DMEM/F12/RPMI1640 (1:1:1) supplemented with EGF and bFGF was used to expand the cells in gel in 96-well plates until the average diameter of cell clusters was about 50-100 μm with the cell density higher than 10(7) cells/mL. In the second step, t...

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Publication status:
Published

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Publisher copy:
10.1007/s12010-013-0211-5

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Journal:
Applied biochemistry and biotechnology
Volume:
170
Issue:
2
Pages:
406-419
Publication date:
2013-05-05
DOI:
EISSN:
1559-0291
ISSN:
0273-2289
URN:
uuid:73024d91-7ab2-4d4e-a191-a5432e9787ae
Source identifiers:
405598
Local pid:
pubs:405598

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