Mus81-Eme1 and Rqh1 involvement in processing stalled and collapsed replication forks.

Journal article

The processing of stalled replication forks and the repair of collapsed replication forks are essential functions in all organisms. In fission yeast DNA junctions at stalled replication forks appear to be processed by either the Rqh1 DNA helicase or Mus81-Eme1 endonuclease. Accordingly, we show that the hypersensitivity to agents that cause replication fork stalling of mus81, eme1, and rqh1 mutants is suppressed by a Holliday junction resolvase (RusA), as is the synthetic lethality of a mus81...

Expand abstract

Authors: Doe, C; Ahn, J; Dixon, J; Whitby, M

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 277
• Issue: 36
• Pages: 32753-32759
• Publication date: 2002-09-01
• DOI: 10.1074/jbc.m202120200
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Schizosaccharomyces pombe Proteins; Plasmids; Substrate Specificity; Holliday Junction Resolvases; Dose-Response Relationship, Drug; Camptothecin; Endodeoxyribonucleases; Saccharomyces cerevisiae Proteins; Recombinant Proteins; DNA Replication; DNA; Endonucleases; DNA-Binding Proteins; Escherichia coli Proteins; DNA Helicases; Mutation; Escherichia coli; Cell Nucleus; Ultraviolet Rays