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In situ assay for analyzing the chromatin binding of proteins in fission yeast.

Abstract:

An in situ technique for studying the chromatin binding of proteins in single fission yeast cells (Schizosaccharomyces pombe) is described. Cells are permeabilized by enzymatic digestion and extracted with a detergent-containing buffer. This procedure removes soluble proteins, but proteins that are bound to insoluble cell structures such as chromatin are retained, and overall cell morphology is maintained. Extraction of proteins is monitored by fluorescence microscopy, either using fluorescen...

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Institution:
University of Oxford
Department:
Oxford, MPLS, Zoology
Brimage, L More by this author
Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
296
Pages:
181-188
Publication date:
2005
EISSN:
1940-6029
ISSN:
1064-3745
URN:
uuid:7244e6cb-71b3-4fb4-8785-57afb8f5aa1a
Source identifiers:
209359
Local pid:
pubs:209359

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