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Structural insight into industrially-relevant glucoamylases: flexible positions of startch-binding domains

Abstract:

Glucoamylases (GA’s) are one of the most important classes of enzymes in the industrial degradation of starch biomass. They consist of a catalytic domain and a carbohydrate binding domain (CBM), with the latter being important for the interaction with polymeric substrate. Whereas the catalytic mechanism and the structure of the individual domains are well known, the spatial arrangement of the domains with each other and its influence on activity are not fully understood. We have crystallised ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1107/S2059798318004989

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
ORCID:
0000-0003-4364-823X
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Publisher:
International Union of Crystallography Publisher's website
Journal:
Acta Crystallographica Section D: Biological Crystallography Journal website
Volume:
74
Issue:
5
Pages:
463-470
Publication date:
2018-04-26
Acceptance date:
2018-03-27
DOI:
EISSN:
1399-0047
ISSN:
0907-4449
Pubs id:
pubs:831924
URN:
uri:71b9d409-1985-45e4-a21f-404901eaee54
UUID:
uuid:71b9d409-1985-45e4-a21f-404901eaee54
Local pid:
pubs:831924

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