Journal article

Structures of the O-linked oligosaccharides of a complex glycoconjugate from Pseudallescheria boydii.

Abstract:: Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline beta-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was characterized by a combination of techniques including electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI MS/MS), matrix-assisted laser desorption ionization mass spectrometry (MALDI MS), nuclear magnetic resonance (NMR), and methylation gas-liquid chromatography-mass spectrometry (GC-MS) analysis. It was branched, with a principal chain of alpha-Rhap-(1 --> 3)-alpha-Rhap-(1 --> 3)-alpha-Manp-(1 --> 2)-Man-ol substituted at O-6 of mannitol with an alpha-Glcp-(1 --> 4)-beta-Galp group. Species containing one and two additional alpha-Glcp-(1 --> 4) substituents in the rhamnose branch were also present. The major component of fraction 2 was a substructure of oligosaccharide-1, lacking a hexose from the Glc-Gal branch. Fraction 3 contained a mixture of smaller, unbranched, oligosaccharides. In hapten inhibition tests, fractions 1 and 2 blocked the reaction between peptidorhamnomannan (PRM) and rabbit anti-P. boydii mycelium hyperimmune serum by approximately 75%, whereas fraction 3 inhibited by approximately 55%.

Publication status:: Published

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APA Style

Pinto, M. R., Gorin, P., Wait, R., Mulloy, B., & Barreto-Bergter, E. (2005). Structures of the O-linked oligosaccharides of a complex glycoconjugate from Pseudallescheria boydii. Glycobiology, 15(10), 895–904.

MLA Style

Pinto, M. R., et al. “Structures of the O-Linked Oligosaccharides of a Complex Glycoconjugate from Pseudallescheria Boydii.” Glycobiology, vol. 15, no. 10, 2005, pp. 895–904.

Chicago Style

Pinto, MR, P Gorin, R Wait, B Mulloy, and E Barreto-Bergter. 2005. “Structures of the O-Linked Oligosaccharides of a Complex Glycoconjugate from Pseudallescheria Boydii.” Glycobiology 15 (10): 895–904.
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Publisher copy:: 10.1093/glycob/cwi084

Authors

+ Pinto, MR More by this author

Role:: Author

+ Gorin, P More by this author

Role:: Author

+ Wait, R More by this author

Institution:: University of Oxford
Division:: MSD
Department:: NDORMS
Role:: Author

+ Mulloy, B More by this author

Role:: Author

+ Barreto-Bergter, E More by this author

Role:: Author

Journal:: Glycobiology More from this journal
Volume:: 15
Issue:: 10
Pages:: 895-904
Publication date:: 2005-10-01
DOI:: 10.1093/glycob/cwi084
EISSN:: 1460-2423
ISSN:: 0959-6658

Language:: English
Keywords:: Immune Sera

Spectrometry, Mass, Electrospray Ionization

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Glycoconjugates

Methylation

Rabbits

Oligosaccharides, Branched-Chain

Animals

Gas Chromatography-Mass Spectrometry

Magnetic Resonance Spectroscopy

Pseudallescheria
Pubs id:: pubs:230209
UUID:: uuid:71a76d0a-7b83-43ee-9c4b-b74a043d8c9d
Local pid:: pubs:230209
Source identifiers:: 230209
Deposit date:: 2013-11-16

Terms of use

Copyright date:: 2005

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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