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A three-colour flow cytometry technique for measuring trophoblast intracellular antigens: the relative expression of TAP1 in human cytotrophoblast and decidual cells.

Abstract:

Flow cytometry is conventionally used to measure cell-surface antigen expression. However, many antigens are found within the cytoplasm, and it is necessary to fix and permeabilize cells to enable antibodies to gain access to them. In this study we have established the conditions for studying intracellular antigens in human trophoblast cells by flow cytometry using an antibody to TAP1 (a key molecule in the process of Class I MHC assembly). We have previously shown by immunocytochemistry that...

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Publication status:
Published

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Publisher copy:
10.1053/plac.2000.0583

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Institution:
University of Oxford
Department:
Oxford, MSD, Obstetrics and Gynaecology
Role:
Author
Journal:
Placenta
Volume:
21
Issue:
8
Pages:
743-753
Publication date:
2000-11-05
DOI:
EISSN:
1532-3102
ISSN:
0143-4004
URN:
uuid:70f8f6cb-fc62-4265-8107-ce07c62f636b
Source identifiers:
248176
Local pid:
pubs:248176

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