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Purification and assembly in vitro of tubulin from Trypanosoma brucei brucei.

Abstract:

Trypanosome tubulin was purified to near homogeneity by chromatography on DEAE-Sephadex, Amicon filtration and assembly-disassembly in vitro. Polymerization of the tubulin in vitro yielded long, structurally normal, microtubules and some sheet structures on addition of GTP and incubation at 37 degrees C, in either the presence or the absence of Mg2+. Tubulin assembly was disrupted by glycerol and a selection of microtubule-reactive drugs. Immunological analysis of the purified tubulin reveale...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
The Biochemical journal
Volume:
265
Issue:
1
Pages:
87-93
Publication date:
1990-01-05
EISSN:
1470-8728
ISSN:
0264-6021
URN:
uuid:70ea470c-1ae0-483d-805a-c77a8686c976
Source identifiers:
25105
Local pid:
pubs:25105

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