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Journal article

Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells.

Abstract:

We present a novel and efficient non-integrating gene expression system in human embryonic stem cells (hESc) utilizing human artificial chromosomes (HAC), which behave as autonomous endogenous host chromosomes and segregate correctly during cell division. HAC are important vectors for investigating the organization and structure of the kinetochore, and gene complementation. HAC have so far been obtained in immortalized or tumour-derived cell lines, but never in stem cells, thus limiting their...

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Publication status:
Published

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Publisher copy:
10.1093/hmg/ddr144

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Journal:
Human molecular genetics
Volume:
20
Issue:
15
Pages:
2905-2913
Publication date:
2011-08-05
DOI:
EISSN:
1460-2083
ISSN:
0964-6906
URN:
uuid:70b84267-f7d4-49b9-ae79-ede00a943052
Source identifiers:
141152
Local pid:
pubs:141152

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