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A toolkit enabling efficient, scalable and reproducible gene tagging in trypanosomatids.

Abstract:

One of the first steps in understanding a protein's function is to determine its localization; however, the methods for localizing proteins in some systems have not kept pace with the developments in other fields, creating a bottleneck in the analysis of the large datasets that are generated in the post-genomic era. To address this, we developed tools for tagging proteins in trypanosomatids. We made a plasmid that, when coupled with long primer PCR, can be used to produce transgenes at their ...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1098/rsob.140197

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Hodkinson, I More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
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Funding agency for:
Dean, S
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Funding agency for:
Wheeler, RJ
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Funding agency for:
Gluenz, E
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Funding agency for:
Gull, K
Publisher:
Royal Society Publisher's website
Journal:
Open Biology Journal website
Volume:
5
Issue:
1
Pages:
Article: 140197
Publication date:
2015
DOI:
EISSN:
2046-2441
ISSN:
2046-2441
URN:
uuid:709754f2-c2d3-4474-a410-e8fb7b9bfe57
Source identifiers:
503190
Local pid:
pubs:503190

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