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Evaluation of the deuterium isotope effect in zwitterionic hydrophilic interaction liquid chromatography separations for implementation in a quantitative proteomic approach.

Abstract:

Quantitative methodologies for the global in-depth comparison of proteomes are frequently based on chemical derivatization of peptides with isotopically distinguishable labeling agents. In the present work, we set out to study the feasibility of the dimethyl labeling method in combination with ZIC-cHILIC (zwitterionic hydrophilic interaction liquid chromatography) technology for quantitative proteomics. We first addressed the potential issue of isotope effects perturbing the essential coeluti...

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Publication status:
Published

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Publisher copy:
10.1021/ac2018074

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Role:
Author
Journal:
Analytical chemistry
Volume:
83
Issue:
21
Pages:
8352-8356
Publication date:
2011-11-05
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
URN:
uuid:70963d92-9304-4cee-aacd-691fa7b4e75c
Source identifiers:
384561
Local pid:
pubs:384561

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