Immunogold electron microscopic localization of timothy grass (Phleum pratense) pollen major allergens Phl p I and Phl p V after anhydrous fixation in acrolein vapor.

Journal article

We used the vapor phase of acrolein as an anhydrous fixative for timothy grass pollen in an immunogold double-labeling localization study of two different major allergens, Phl p I and Phl p V. More than 48 hr of fixation were needed for the subcellular pollen structures to be satisfactorily stabilized. The immunoreactivity of acrolein-fixed pollen allergens was not destroyed even after prolonged acrolein fixation. By immunoblotting, the two allergens differ in their immunological and structur...

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Authors: Grote, M; Dolecek, C; Van Ree, R; Valenta, R

• Publication status: Published
• Journal: journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
• Volume: 42
• Issue: 3
• Pages: 427-431
• Publication date: 1994-03-01
• DOI: 10.1177/42.3.8308259
• EISSN: 1551-5044
• ISSN: 0022-1554
• Language: English
• Keywords: Gases; Animals; Acrolein; Poaceae; Immunohistochemistry; Mice; Tissue Fixation; Antibodies, Monoclonal; Pollen; Microscopy, Immunoelectron; Immunoblotting; Allergens; Fixatives; Rabbits