- Abstract:
-
Biological membranes decorated with suitable contrast agents give rise to nonlinear optical signals such as two-photon fluorescence and harmonic up-conversion when illuminated with ultra-short, high-intensity pulses of infrared laser light. Microscopic images based on these nonlinear contrasts were acquired at video or higher frame rates by scanning a focused illuminating spot rapidly across neural tissues. The scan engine relied on an acousto-optic deflector (AOD) to produce a fast horizonta...
Expand abstract - Publisher copy:
- 10.1152/jn.00087.2004
-
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- Journal:
- Journal of neurophysiology
- Volume:
- 92
- Issue:
- 1
- Pages:
- 609-621
- Publication date:
- 2004-07-05
- DOI:
- EISSN:
-
1522-1598
- ISSN:
-
0022-3077
- URN:
-
uuid:6fab158b-7256-43cd-8852-93300339cf8b
- Source identifiers:
-
322563
- Local pid:
- pubs:322563
- Copyright date:
- 2004
Journal article
Video-rate nonlinear microscopy of neuronal membrane dynamics with genetically encoded probes.
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