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Journal article

Video-rate nonlinear microscopy of neuronal membrane dynamics with genetically encoded probes.

Abstract:

Biological membranes decorated with suitable contrast agents give rise to nonlinear optical signals such as two-photon fluorescence and harmonic up-conversion when illuminated with ultra-short, high-intensity pulses of infrared laser light. Microscopic images based on these nonlinear contrasts were acquired at video or higher frame rates by scanning a focused illuminating spot rapidly across neural tissues. The scan engine relied on an acousto-optic deflector (AOD) to produce a fast horizonta...

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Publisher copy:
10.1152/jn.00087.2004

Authors


Journal:
Journal of neurophysiology
Volume:
92
Issue:
1
Pages:
609-621
Publication date:
2004-07-05
DOI:
EISSN:
1522-1598
ISSN:
0022-3077
URN:
uuid:6fab158b-7256-43cd-8852-93300339cf8b
Source identifiers:
322563
Local pid:
pubs:322563

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