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Incorporation of oxygen into the succinate co-product of iron(II) and 2-oxoglutarate dependent oxygenases from bacteria, plants and humans.

Abstract:

The ferrous iron and 2-oxoglutarate (2OG) dependent oxygenases catalyse two electron oxidation reactions by coupling the oxidation of substrate to the oxidative decarboxylation of 2OG, giving succinate and carbon dioxide coproducts. The evidence available on the level of incorporation of one atom from dioxygen into succinate is inconclusive. Here, we demonstrate that five members of the 2OG oxygenase family, AlkB from Escherichia coli, anthocyanidin synthase and flavonol synthase from Arabido...

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Publication status:
Published

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Journal:
FEBS letters
Volume:
579
Issue:
23
Pages:
5170-5174
Publication date:
2005-09-05
DOI:
EISSN:
1873-3468
ISSN:
0014-5793
URN:
uuid:6f9825cd-1f5f-4e0d-b4d0-73dbc623f52f
Source identifiers:
39644
Local pid:
pubs:39644

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