Mutation of GOGAT prevents pea bacteroid formation and N2 fixation by globally downregulating transport of organic nitrogen sources.

Journal article

Mutation of gltB (encoding glutamate oxoglutarate amidotransferase or GOGAT) in RU2307 increased the intracellular Gln:Glu ratio and inhibited amino acid transport via Aap and Bra. The mechanism probably involves global post-translational inhibition independent of Ntr. Transport was separately restored by increased gene expression of Aap or heterologous transporters. Likewise, second site suppressor mutations in the RNA chaperone Hfq elevated transport by Aap and Bra by increasing mRNA levels...

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Authors: Mulley, G; White, J; Karunakaran, R; Prell, J; Bourdes, A

• Journal: Molecular microbiology
• Volume: 80
• Issue: 1
• Pages: 149-167
• Publication date: 2011-04-01
• DOI: 10.1111/j.1365-2958.2011.07565.x
• EISSN: 1365-2958
• ISSN: 0950-382X
• Language: English
• Keywords: Mass Spectrometry; Rhizobium leguminosarum; Chromatography, Liquid; Nitrogen; Peas; Mutation; Nitrogen Fixation; Bacterial Proteins; Reverse Transcriptase Polymerase Chain Reaction