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Generation of baculovirus vectors for the high-throughput production of proteins in insect cells.

Abstract:

The baculovirus expression system is one of the most popular methods used for the production of recombinant proteins but has several complex steps which have proved inherently difficult to adapt to a multi-parallel process. We have developed a bacmid vector that does not require any form of selection pressure to separate recombinant virus from non-recombinant parental virus. The method relies on homologous recombination in insect cells between a transfer vector containing a gene to be express...

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Publication status:
Published

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Publisher copy:
10.1002/bit.22002

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Journal:
Biotechnology and bioengineering More from this journal
Volume:
101
Issue:
6
Pages:
1115-1122
Publication date:
2008-12-01
DOI:
EISSN:
1097-0290
ISSN:
0006-3592
Language:
English
Keywords:
Pubs id:
pubs:34380
UUID:
uuid:6f3d71ad-f9a9-41e4-add0-206433e6b06d
Local pid:
pubs:34380
Source identifiers:
34380
Deposit date:
2012-12-19

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