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Sensorless adaptive optics for isoSTED nanoscopy

Abstract:

The presence of aberrations is a major concern when using fluorescence microscopy to image deep inside tissue. Aberrations due to refractive index mismatch and heterogeneity of the specimen under investigation cause severe reduction in the amount of fluorescence emission that is collected by the microscope. Furthermore, aberrations adversely affect the resolution, leading to loss of fine detail in the acquired images. These phenomena are particularly troublesome for super-resolution microscop...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1117/12.2288973

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Centre for Neural Circuits and Behaviour
Allgeyer, ES More by this author
Bewersdorf, J More by this author
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Institution:
University of Oxford
Division:
MPLS Division
Department:
Engineering Science; Ludwig Institute
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Grant:
095927/A/11/Z, 095927/B/11/Z, 203285/B/16/Z, 203285/C/16/Z
G. Harold and Leila Y. Mathers Foundation More from this funder
Publisher:
Society of Photo-Optical Instrumentation Engineers Publisher's website
Volume:
10502
Pages:
Article: 1050206
Publication date:
2018-02-23
Acceptance date:
2017-09-25
DOI:
ISSN:
1605-7422
Pubs id:
pubs:843451
URN:
uri:6f0befaa-fb0e-4f46-8bc7-0d454b236b3d
UUID:
uuid:6f0befaa-fb0e-4f46-8bc7-0d454b236b3d
Local pid:
pubs:843451
ISBN:
9781510614895

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