Antigen-specific and non-specific helper activities derived from supernatants of human influenza virus-specific T-cell lines.

Journal article

A T-cell line (H3) was established by culturing human peripheral blood mononuclear cells with influenza virus A/X31 and maintained in long term culture with Interleukin-2 (TCGF). Supernatants were prepared by culturing these cells overnight in the absence of Interleukin-2 but with A/X31 and irradiated autologous E rosette negative cells as a source of antigen presenting cells, and harvesting by centrifugation. The supernatants were shown to replace T cells in helping E- (B) cells to produce a...

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Authors: Zanders, E; Fischer, A; Smith, S; Beverley, P; Feldmann, M

• Publication status: Published
• Journal: Immunology
• Volume: 48
• Issue: 2
• Pages: 361-366
• Publication date: 1983-02-01
• EISSN: 1365-2567
• ISSN: 0019-2805
• Language: English
• Keywords: B-Lymphocytes; Humans; Lymphocyte Cooperation; Molecular Weight; Cell Line; Orthomyxoviridae; Chromatography, Gel; T-Lymphocytes; Genes, MHC Class II; Antibodies, Viral; Cell-Free System