Comparison of the sensitivity of in vivo and in vitro assays for detection of antiviral cytotoxic T cell activity.

Journal article

Sensitivities of in vitro and in vivo assays for the detection of lymphocytic choriomeningitis virus (LCMV)-specific CTL were compared. Measurement of primary cytotoxicity was the least sensitive of all the tested assays. However, when the same 51Cr release assays were performed after in vitro restimulation, this in vitro method was found to be more sensitive than all of the five in vivo assays tested. Assessment of CTL-mediated protection against LCMV replication in spleens was most sensitiv...

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Authors: Castelmur, I; DiPaolo, C; Bachmann, M; Hengartner, H; Zinkernagel, R

• Publication status: Published
• Journal: Cellular immunology
• Volume: 151
• Issue: 2
• Pages: 460-466
• Publication date: 1993-10-01
• DOI: 10.1006/cimm.1993.1254
• EISSN: 1090-2163
• ISSN: 0008-8749
• Language: English
• Keywords: Spleen; Immunologic Memory; T-Lymphocytes, Cytotoxic; Mice, Inbred C57BL; Ovary; Edema; Female; Lymphocytic choriomeningitis virus; Virus Replication; Mice; Lymphocytic Choriomeningitis; Animals; Meninges; Cytotoxicity Tests, Immunologic; Sensitivity and Specificity