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Journal article

High-precision correlative fluorescence and electron cryo microscopy using two independent alignment markers.

Abstract:

Correlative light and electron microscopy (CLEM) is an emerging technique which combines functional information provided by fluorescence microscopy (FM) with the high-resolution structural information of electron microscopy (EM). So far, correlative cryo microscopy of frozen-hydrated samples has not reached better than micrometre range accuracy. Here, a method is presented that enables the correlation between fluorescently tagged proteins and electron cryo tomography (cryoET) data with nanome...

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Publication status:
Published

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Publisher:
Elsevier
Journal:
Ultramicroscopy More from this journal
Volume:
143
Pages:
41-51
Publication date:
2014-08-01
DOI:
EISSN:
1879-2723
ISSN:
0304-3991
Language:
English
Keywords:
Pubs id:
pubs:440165
UUID:
uuid:6e890c42-13e5-4635-8f78-74ee9f24b840
Local pid:
pubs:440165
Source identifiers:
440165
Deposit date:
2013-12-11

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