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The detection by immunofluorescence of distinct cell populations producing interleukin-1 alpha and interleukin-1 beta in activated human peripheral blood.

Abstract:

An immunofluorescent staining method using specific monoclonal antibodies was used to detect IL-1 alpha and IL-1 beta in individual cells in stimulated human peripheral blood. No staining was seen in unstimulated cells but intense, maximal staining of approximately 5% of the cells was seen 20-24 h after activation with PHA/PMA. The large irregularly shaped stained cells were surrounded by smaller unstained cells with lymphocyte-like morphology. By 44 h post activation a few cells only showed ...

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Publication status:
Published

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Institution:
University of Oxford
Division:
MSD
Department:
NDORMS
Role:
Author
Journal:
Journal of immunological methods More from this journal
Volume:
120
Issue:
2
Pages:
277-283
Publication date:
1989-06-01
DOI:
EISSN:
1872-7905
ISSN:
0022-1759
Language:
English
Keywords:
Pubs id:
pubs:481765
UUID:
uuid:6e567573-0c88-4530-8d50-22022523da2e
Local pid:
pubs:481765
Source identifiers:
481765
Deposit date:
2014-08-30

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