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Protein-protein interactions and metabolite channelling in the plant tricarboxylic acid cycle

Abstract:

Protein complexes of sequential metabolic enzymes, often termed metabolons, may permit direct channeling of metabolites between the enzymes, providing increased control over metabolic pathway fluxes. Experimental evidence supporting their existence in vivo remains fragmentary. In the present study, binary interactions of the proteins constituting the plant tricarboxylic acid (TCA) cycle are tested. Affinity purification-mass spectrometry, split-luciferase and yeast-two-hybrid assays are emplo...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/ncomms15212

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Plant Sciences
Role:
Author
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Funding agency for:
Beard, K
Grant:
CASE studentship
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Funding agency for:
Fernie, A
Grant:
FP7 Project ERA-NET Plant Genomics, STRESSNET
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Funding agency for:
Obata, T
Grant:
FP7 Project ERA-NET Plant Genomics, STRESSNET
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Publisher:
Nature Publishing Group Publisher's website
Journal:
Nature Communications Journal website
Volume:
8
Issue:
15212
Pages:
1-11
Publication date:
2017-05-16
Acceptance date:
2017-03-09
DOI:
EISSN:
2041-1723
Source identifiers:
686363
Pubs id:
pubs:686363
UUID:
uuid:6e291b22-d4fc-4fe9-9718-5d5712e1e21d
Local pid:
pubs:686363
Deposit date:
2017-03-20

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