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Quadrupole-time-of-flight mass spectrometer modified for higher-energy dissociation reduces protein assemblies to peptide fragments

Abstract:

The gas-phase dissociation of protein assemblies is becoming crucial for the application of mass spectrometry to structural biology. However certain aspects of the dissociation mechanism remain elusive. Moreover, many protein complexes resist dissociation at the energies accessible with current instrumentation. Here we report new insights into the collision-induced dissociation mechanism of protein assemblies. By holding activation energy constant and varying the charge state of the precursor...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1021/ac801950u

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Department:
University College
Role:
Author
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Department:
Exeter College
Role:
Author
Publisher:
American Chemical Society Publisher's website
Journal:
Analytical chemistry Journal website
Volume:
81
Issue:
3
Pages:
1270-1274
Publication date:
2009-02-01
DOI:
ISSN:
1520-6882 and 0003-2700
Pubs id:
pubs:59319
URN:
uri:6e03f5d2-842d-456b-8c33-2362950e3756
UUID:
uuid:6e03f5d2-842d-456b-8c33-2362950e3756
Local pid:
pubs:59319
Language:
English
Keywords:

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