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RNA sequencing (RNA-Seq) reveals extremely low levels of reticulocyte-derived globin gene transcripts in peripheral blood from horses (Equus caballus) and cattle (Bos taurus)

Abstract:

RNA-seq has emerged as an important technology for measuring gene expression in peripheral blood samples collected from humans and other vertebrate species. In particular, transcriptomics analyses of whole blood can be used to study immunobiology and develop novel biomarkers of infectious disease. However, an obstacle to these methods in many mammalian species is the presence of reticulocyte-derived globin mRNAs in large quantities, which can complicate RNA-seq library sequencing and impede d...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.3389/fgene.2018.00278

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Grant:
Framework 7 Project Grant (No: KBBE-211602- MACROSYS)
Publisher:
Frontiers Media Publisher's website
Journal:
Frontiers in Genetics Journal website
Volume:
9
Issue:
AUG
Pages:
Article: 278
Publication date:
2018-08-14
Acceptance date:
2018-07-09
DOI:
EISSN:
1664-8021
Pubs id:
pubs:911118
URN:
uri:6dc30b5e-d444-461c-89f1-cb41d3fe1e6c
UUID:
uuid:6dc30b5e-d444-461c-89f1-cb41d3fe1e6c
Local pid:
pubs:911118

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