- Abstract:
-
Potential factors influencing antigen detection in immunoassays for measuring rat or mouse aeroallergen (i.e., assay setup, antigen specificities, standard extracts used, and antigen decay) were investigated in a three-country study (the UK, The Netherlands, Sweden). An inhibition enzyme immunoassay (EIA) setup gave nominal rat urinary allergen (RUA) sample values seven times higher than a sandwich EIA setup utilizing identical antibodies and standards. In immunoblotting experiments, pooled p...
Expand abstract - Publisher copy:
- 10.1034/j.1398-9995.1999.00631.x
-
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- Journal:
- Allergy
- Volume:
- 54
- Issue:
- 2
- Pages:
- 150-157
- Publication date:
- 1999-02-05
- DOI:
- EISSN:
-
1398-9995
- ISSN:
-
0105-4538
- URN:
-
uuid:6db0d629-c267-4399-9df0-f1478000048a
- Source identifiers:
-
245613
- Local pid:
- pubs:245613
- Language:
- English
- Keywords:
- Copyright date:
- 1999
Journal article
Comparison of methods to assess airborne rat and mouse allergen levels. II. Factors influencing antigen detection.
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