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Comparison of methods to assess airborne rat and mouse allergen levels. II. Factors influencing antigen detection.

Abstract:

Potential factors influencing antigen detection in immunoassays for measuring rat or mouse aeroallergen (i.e., assay setup, antigen specificities, standard extracts used, and antigen decay) were investigated in a three-country study (the UK, The Netherlands, Sweden). An inhibition enzyme immunoassay (EIA) setup gave nominal rat urinary allergen (RUA) sample values seven times higher than a sandwich EIA setup utilizing identical antibodies and standards. In immunoblotting experiments, pooled p...

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Journal:
Allergy
Volume:
54
Issue:
2
Pages:
150-157
Publication date:
1999-02-01
DOI:
EISSN:
1398-9995
ISSN:
0105-4538
Source identifiers:
245613
Language:
English
Keywords:
Pubs id:
pubs:245613
UUID:
uuid:6db0d629-c267-4399-9df0-f1478000048a
Local pid:
pubs:245613
Deposit date:
2012-12-19

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