Validating the location of fluorescent protein fusions in the endomembrane system.

Journal article

Assessment of gene function generally requires knowledge of the sites of action of gene products. Several experimental approaches can provide relevant information, but all have their limitations and the potential for experimental artifact. In this article we focus on the endomembrane organelles and on the methods that can be used to validate the location of fluorescent protein fusions. We discuss the utility of redundant localization techniques, complementation of mutant phenotypes, and integ...

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Authors: Moore, I; Murphy, A

• Publication status: Published
• Journal: Plant cell
• Volume: 21
• Issue: 6
• Pages: 1632-1636
• Publication date: 2009-06-01
• DOI: 10.1105/tpc.109.068668
• EISSN: 1532-298X
• ISSN: 1040-4651
• Language: English
• Keywords: Mutation; Research Design; Organelles; Luminescent Proteins; Immunohistochemistry; Intracellular Membranes; Phenotype; Recombinant Fusion Proteins; Plant Proteins; Electrophoresis, Polyacrylamide Gel; Genetic Complementation Test