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Characterization of biases in phosphopeptide enrichment by Ti(4+)-immobilized metal affinity chromatography and TiO2 using a massive synthetic library and human cell digests.

Abstract:

Outcomes of comparative evaluations of enrichment methods for phosphopeptides depend highly on the experimental protocols used, the operator, the source of the affinity matrix, and the samples analyzed. Here, we attempt such a comparative study exploring a very large synthetic library containing thousands of serine, threonine, and tyrosine phosphorylated peptides, being present in roughly equal abundance, along with their nonphosphorylated counterparts, and use an optimized protocol for enric...

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Publication status:
Published

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Publisher copy:
10.1021/ac501803z

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
Journal:
Analytical chemistry More from this journal
Volume:
86
Issue:
16
Pages:
8312-8320
Publication date:
2014-08-04
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
Language:
English
Keywords:
Pubs id:
pubs:480136
UUID:
uuid:6d52dd21-ec36-4d7d-a408-9b1708409174
Local pid:
pubs:480136
Source identifiers:
480136
Deposit date:
2014-08-17

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