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Using the fluorescent properties of STO-609 as a tool to assist structure-function analyses of recombinant CaMKK2.

Abstract:

Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in the regulation of metabolic activity in cancer and immune cells, and affects whole-body metabolism by regulating ghrelinsignalling in the hypothalamus. This has led to efforts to develop specific CaMKK2 inhibitors, and STO- 609 is the standardly used CaMKK2 inhibitor to date. We have developed a novel fluorescence-based assay by exploiting the intrinsic fluorescence properties of STO-609. Here, we report an in vit...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1016/j.bbrc.2016.05.045

Authors


Temmerman, K More by this author
Lawrence-Dörner, A-M More by this author
More by this author
ORCID:
0000-0002-7249-4762
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Norwegian Research Council More from this funder
Southern and Eastern Norway Regional Health Authority More from this funder
University of Oslo More from this funder
European Molecular Biology Laboratory More from this funder
Cancer Research UK More from this funder
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Publisher:
Elsevier Publisher's website
Journal:
Biochemical and Biophysical Research Communications Journal website
Volume:
476
Issue:
2
Pages:
102-107
Publication date:
2016-05-05
Acceptance date:
2016-05-09
DOI:
EISSN:
1090-2104
ISSN:
0006-291X
Pubs id:
pubs:817488
URN:
uri:6d314936-2e81-4dc5-b23f-555efe0b0092
UUID:
uuid:6d314936-2e81-4dc5-b23f-555efe0b0092
Local pid:
pubs:817488

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