Adenovirus hexon protein enhances nuclear delivery and increases transgene expression of polyethylenimine/plasmid DNA vectors.

Journal article

Inefficient nuclear delivery restricts transgene expression using polyelectrolyte DNA vectors. To increase transfer from the cytoplasm to the nucleus, we have covalently linked adenovirus hexon protein to polyethylenimine (PEI, 800 kDa). Activity of the conjugate was compared with PEI and PEI linked to albumin. Hexon-containing complexes gave 10-fold greater transgene expression in HepG2 cells than PEI/DNA or complexes containing albumin, without increasing cell uptake. Following cytoplasmic ...

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Authors: Carlisle, R; Bettinger, T; Ogris, M; Hale, S; Mautner, V

• Publication status: Published
• Journal: Molecular therapy : the journal of the American Society of Gene Therapy
• Volume: 4
• Issue: 5
• Pages: 473-483
• Publication date: 2001-11-01
• DOI: 10.1006/mthe.2001.0472
• EISSN: 1525-0024
• ISSN: 1525-0016
• Language: English
• Keywords: Plasmids; Rabbits; Serum Albumin, Bovine; Animals; Active Transport, Cell Nucleus; Rats; Tumor Cells, Cultured; Gene Expression; Fibroblasts; Cell Nucleus; Microscopy, Electron; Xenopus laevis; Transfection; Transgenes; Gene Therapy; Cattle; Nuclear Localization Signals; Genetic Vectors; Capsid; Microinjections; Oocytes; Polyethyleneimine; Humans; Capsid Proteins; Fluorescein-5-isothiocyanate