Confocal fluorescence imaging of photosensitized DNA denaturation in cell nuclei.

Journal article

The double-stranded helical structure of DNA is maintained in part by hydrogen bonds between strands and by stacking interactions between adjacent purine and pyrimidine bases in one strand. The transition (denaturation) from a double-stranded (ds) to a single-stranded (ss) form can be induced in isolated DNA or fixed cells by exposure to elevated temperatures, alkali or acids, aprotic or nonpolar solvents or some drugs. We report here that DNA denaturation can occur in situ in cell nuclei as ...

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Authors: Bernas, T; Asem, E; Robinson, J; Cook, P; Dobrucki, J

• Publication status: Published
• Journal: Photochemistry and photobiology
• Volume: 81
• Issue: 4
• Pages: 960-969
• Publication date: 2005-01-01
• DOI: 10.1562/2004-11-11-ra-369r.1
• EISSN: 1751-1097
• ISSN: 0031-8655
• Language: English
• Keywords: Cell Nucleus; DNA; Humans; Photosensitizing Agents; Cell Line; Fibroblasts; Nucleic Acid Denaturation; Nucleic Acid Conformation