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Composition and structure of synaptic ectosomes exporting antigen receptor linked to functional CD40 ligand from helper T-cells

Abstract:

Planar supported lipid bilayers (PSLBs) presenting T cell receptor (TCR) ligands and ICAM-1 induce budding of extracellular microvesicles enriched in functional TCR, defined here as synaptic ectosomes (SE), from helper T cells. SE bind peptide-MHC directly exporting TCR into the synaptic cleft, but incorporation of other effectors is unknown. Here, we utilized bead supported lipid bilayers (BSLB) to capture SE from single immunological synapses (IS), determined SE composition by immunofluores...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.7554/elife.47528

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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDORMS
Sub department:
Kennedy Institute of Rheumatology
Role:
Author
More by this author
Role:
Author
ORCID:
0000-0002-1641-4107
More by this author
Role:
Author
ORCID:
0000-0003-4470-5881
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDORMS
Sub department:
Kennedy Institute of Rheumatology
Role:
Author
ORCID:
0000-0002-3050-7633
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDORMS
Sub department:
Kennedy Institute of Rheumatology
Role:
Author
Kennedy Institute of Rheumatology Trust More from this funder
Publisher:
eLife Sciences Publications Publisher's website
Journal:
eLife
Volume:
8
Article number:
e47528
Publication date:
2019-08-30
Acceptance date:
2019-08-28
DOI:
EISSN:
2050-084X
Language:
English
Keywords:
Pubs id:
pubs:1048548
UUID:
uuid:6cf62516-0e90-4726-a736-a8b0c6301127
Local pid:
pubs:1048548
Source identifiers:
1048548
Deposit date:
2019-09-02

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