Exploiting the carboxylate chemical shift to resolve degenerate resonances in spectra of 13C-labelled glycosaminoglycans.

Journal article

Glycosaminoglycans (GAG) are important vertebrate extracellular matrix polysaccharides that comprise repeated units of an acidic and an N-acetylated sugar. The constituent acidic sugars are central to their biological functions, but have been largely inaccessible to NMR because the (1)H resonances overlap with those from other residues. Here, pulse sequences that address this failure are developed using (13)C-enriched oligosaccharides of the glycosaminoglycan, hyaluronan, as model systems. Tw...

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Authors: Colebrooke, SA; Blundell, C; DeAngelis, P; Campbell, I; Almond, A

• Journal: Magnetic resonance in chemistry : MRC
• Volume: 43
• Issue: 10
• Pages: 805-815
• Publication date: 2005-10-01
• DOI: 10.1002/mrc.1620
• EISSN: 1097-458X
• ISSN: 0749-1581
• Language: English
• Keywords: Carbohydrate Conformation; Molecular Sequence Data; Glycosaminoglycans; Carbon Isotopes; Reference Standards; Hyaluronic Acid; Magnetic Resonance Spectroscopy; Carbohydrate Sequence; Carboxylic Acids; Sensitivity and Specificity