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Multicenter assessment of animal-free collagenase AF-1 for human islet isolation

Abstract:

Animal-free (AF) SERVA Collagenase AF-1 and Neutral Protease (NP) AF GMP Grade have recently become available for human islet isolation. This report describes the initial experiences of 3 different islet transplant centers. Thirty-four human pancreases were digested using 1 vial of the 6 different lots of Collagenase AF-1 (2,000-2,583 PZ-U/vial) supplemented with 4 different lots of NP AF in a range of 50 to 160 DMC-U per pancreas. Isolation, culture, and quality assessment were performed usi...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1177/0963689717731574

Authors


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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Surgical Sciences
Parnaud, G More by this author
Friberg, A More by this author
Lavallard, V More by this author
Demuylder-Mischler, S More by this author
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Diabetes Wellness Foundation More from this funder
Oxford Biomedical Research Centre More from this funder
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Publisher:
SAGE Publications Publisher's website
Journal:
Cell Transplantation Journal website
Volume:
26
Issue:
10
Pages:
1688-1693
Publication date:
2017-12-18
Acceptance date:
2017-08-21
DOI:
ISSN:
0963-6897 and 1555-3892
Pubs id:
pubs:811730
URN:
uri:6c97ebb1-9d55-424f-aef8-787dd5d7bf33
UUID:
uuid:6c97ebb1-9d55-424f-aef8-787dd5d7bf33
Local pid:
pubs:811730

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