- Abstract:
-
Animal-free (AF) SERVA Collagenase AF-1 and Neutral Protease (NP) AF GMP Grade have recently become available for human islet isolation. This report describes the initial experiences of 3 different islet transplant centers. Thirty-four human pancreases were digested using 1 vial of the 6 different lots of Collagenase AF-1 (2,000-2,583 PZ-U/vial) supplemented with 4 different lots of NP AF in a range of 50 to 160 DMC-U per pancreas. Isolation, culture, and quality assessment were performed usi...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's version
- Files:
-
-
(pdf, 300.8kb)
-
- Publisher copy:
- 10.1177/0963689717731574
-
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- Publisher:
- SAGE Publications Publisher's website
- Journal:
- Cell Transplantation Journal website
- Volume:
- 26
- Issue:
- 10
- Pages:
- 1688-1693
- Publication date:
- 2017-12-18
- Acceptance date:
- 2017-08-21
- DOI:
- ISSN:
-
0963-6897 and 1555-3892
- Pubs id:
-
pubs:811730
- URN:
-
uri:6c97ebb1-9d55-424f-aef8-787dd5d7bf33
- UUID:
-
uuid:6c97ebb1-9d55-424f-aef8-787dd5d7bf33
- Local pid:
- pubs:811730
- Language:
- English
- Keywords:
- Copyright holder:
- © Brandhorst, et al 2017
- Copyright date:
- 2017
- Notes:
-
Creative Commons CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License
(http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further
permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage)
Journal article
Multicenter assessment of animal-free collagenase AF-1 for human islet isolation
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Diabetes Wellness Foundation
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Oxford Biomedical Research Centre
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