- Abstract:
-
Peptide-bound histocompatibility leukocyte antigen (HLA) class I tetramers enable a precise identification of antigen-specific CD8+ T cells using flow cytometry. The combination of this technology with intracellular staining techniques opens up significantly better ways of studying these cells than previously possible, allowing immunologists to look at their life cycle (activation and proliferation), manner of death (aging and apoptosis) and effector function (cytotoxic potential and cytokine...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/s0022-1759(02)00195-3
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- Journal:
- Journal of immunological methods
- Volume:
- 268
- Issue:
- 1
- Pages:
- 9-19
- Publication date:
- 2002-10-05
- DOI:
- EISSN:
-
1872-7905
- ISSN:
-
0022-1759
- URN:
-
uuid:6c5d9743-12c0-4d69-b055-c9f6855df095
- Source identifiers:
-
19403
- Local pid:
- pubs:19403
- Copyright date:
- 2002
Journal article
The assessment of antigen-specific CD8+ T cells through the combination of MHC class I tetramer and intracellular staining.
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