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Journal article

Replication fork reversal during DNA interstrand crosslink repair requires CMG unloading

Abstract:

DNA interstrand crosslinks (ICLs) are extremely cytotoxic, but the mechanism of their repair remains incompletely understood. Using Xenopus egg extracts, we previously showed that repair of a cisplatin ICL is triggered when two replication forks converge on the lesion. After CDC45/MCM2-7/GINS (CMG) ubiquitylation and unloading by the p97 segregase, FANCI-FANCD2 promotes DNA incisions by XPF-ERCC1, leading to ICL unhooking. Here, we report that, during this cell-free ICL repair reaction, one o...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's Version

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Publisher copy:
10.1016/j.celrep.2018.05.061

Authors


Amunugama, R More by this author
Willcox, S More by this author
Abdullah, UB More by this author
More by this author
Institution:
University of Oxford
Division:
Maths, Physical & Life Sciences
Department:
Organic Chemistry
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Publisher:
Elsevier Publisher's website
Journal:
Cell reports Journal website
Volume:
23
Issue:
12
Pages:
3419-3428
Publication date:
2018-06-19
Acceptance date:
2018-05-17
DOI:
EISSN:
2211-1247
Pubs id:
pubs:858916
URN:
uri:6c523452-eaef-42ec-b084-f3832ce2fa50
UUID:
uuid:6c523452-eaef-42ec-b084-f3832ce2fa50
Local pid:
pubs:858916

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