Alternate dissociation pathways identified in charge-reduced protein complex ions.

Journal article

Tandem mass spectrometry (MS) of large protein complexes has proven to be capable of assessing the stoichiometry, connectivity, and structural details of multiprotein assemblies. While the utility of tandem MS is without question, a deeper understanding of the mechanism of protein complex dissociation will undoubtedly drive the technology into new areas of enhanced utility and information content. We present here the systematic analysis of the charge state dependent decay of the noncovalently...

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Authors: Pagel, K; Hyung, S; Ruotolo, B; Robinson, C

• Publication status: Published
• Journal: Analytical chemistry
• Volume: 82
• Issue: 12
• Pages: 5363-5372
• Publication date: 2010-06-01
• DOI: 10.1021/ac101121r
• EISSN: 1520-6882
• ISSN: 0003-2700
• Language: English
• Keywords: Ions; Protein Conformation; Protein Multimerization; Humans; Protein Folding; Prealbumin; Mass Spectrometry