In vitro transcription and polymerase binding studies of the termini of influenza A virus cRNA: evidence for a cRNA panhandle.

Journal article

An in vitro transcription assay was used to study transcription from synthetic RNA corresponding to the 3' terminus of influenza A virus cRNA. Micrococcal nuclease-treated influenza virus ribonucleoprotein was used as a source of active polymerase complex. Mutations at two regions of the 13 nucleotide-long conserved cRNA 3' terminus were shown to reduce transcription templated by the short added model RNAs. The first region, at positions 1 and 2 from the 3' terminus, was shown to be affected ...

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Authors: Pritlove, D; Fodor, E; Seong, B; Brownlee, G

• Publication status: Published
• Journal: Journal of general virology
• Volume: 76 ( Pt 9)
• Issue: 9
• Pages: 2205-2213
• Publication date: 1995-09-01
• DOI: 10.1099/0022-1317-76-9-2205
• EISSN: 1465-2099
• ISSN: 0022-1317
• Language: English
• Keywords: Base Sequence; Nucleic Acid Conformation; DNA-Directed RNA Polymerases; Transcription, Genetic; Humans; RNA, Viral; Influenza A virus; Promoter Regions, Genetic; Mutagenesis; Protein Binding; RNA, Complementary; Molecular Sequence Data