A method for the generation of YAC transgenic mice by pronuclear microinjection.

Journal article

Yeast artificial chromosomes (YACs) represent the latest generation of vectors which have the great advantage of large insert size. The introduction of YACs into mammalian cells and organisms has become an important goal, since it offers the potential to study the control of large and complex transcription units and identify genes by complementation. Microinjection into the nucleus is the most direct and efficient way of delivering YAC DNA into cells, but requires the purification of the YAC ...

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Authors: Schedl, A; Larin, Z; Montoliu, L; Thies, E; Kelsey, G

• Publication status: Published
• Journal: Nucleic acids research
• Volume: 21
• Issue: 20
• Pages: 4783-4787
• Publication date: 1993-10-01
• DOI: 10.1093/nar/21.20.4783
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English
• Keywords: DNA, Recombinant; Monophenol Monooxygenase; Transformation, Genetic; Mice, Transgenic; Chromosomes, Artificial, Yeast; Animals; Fertilization; Electrophoresis, Gel, Pulsed-Field; Oocytes; Microinjections; Mice; DNA; Restriction Mapping; Cell Nucleus