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Single molecule fluorescence in situ hybridisation for quantitating post-transcriptional regulation in Drosophila brains.

Abstract:

RNA in situ hybridization is a powerful method to investigate post-transcriptional regulation, but analysis of intracellular mRNA distributions in thick, complex tissues like the brain poses significant challenges. Here, we describe the application of single-molecule fluorescent in situ hybridization (smFISH) to quantitate primary nascent transcription and post-transcriptional regulation in whole-mount Drosophila larval and adult brains. Combining immunofluorescence and smFISH probes for diff...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1016/j.ymeth.2017.06.025

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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Centre for Neural Circuits and Behaviour
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Centre for Neural Circuits and Behaviour
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Clarendon Trust More from this funder
Goodger Fund More from this funder
University College London More from this funder
Biotechnology and Biological Sciences Research Council More from this funder
Fondation Bettencourt Schueller More from this funder
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Publisher:
Elsevier Publisher's website
Journal:
Methods Journal website
Volume:
126
Pages:
166-176
Publication date:
2017-06-24
Acceptance date:
2017-06-21
DOI:
EISSN:
1095-9130
ISSN:
1046-2023
Pubs id:
pubs:702416
URN:
uri:6bb02d29-d0ce-4217-8a68-660da79c1dde
UUID:
uuid:6bb02d29-d0ce-4217-8a68-660da79c1dde
Local pid:
pubs:702416

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