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Dimethyl sulfoxide (DMSO) exposure to human peripheral blood mononuclear cells (PBMCs) abolish T cell responses only in high concentrations and following coincubation for more than two hours.

Abstract:

Immunotherapies based on reinfusion of autologous cells incubated ex vivo with peptides reconstituted in toxic solvents, such as DMSO, are now performed on a routine basis. However, the toxic effects of the most common solvent used, DMSO, on T cell responses from human PBMCs, have not previously been evaluated in detail. Here, in preparation for a first-in-man human phase I vaccine trial comprising reinfusion of autologous HIV peptide-pulsed PBMCs, human PBMCs from healthy and HIV-infected do...

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Publication status:
Published

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Publisher copy:
10.1016/j.jim.2010.01.014

Authors


Kloverpris, H More by this author
Fomsgaard, A More by this author
Handley, A More by this author
Ackland, J More by this author
Sullivan, M More by this author
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Journal:
Journal of immunological methods
Volume:
356
Issue:
1-2
Pages:
70-78
Publication date:
2010-04-05
DOI:
EISSN:
1872-7905
ISSN:
0022-1759
URN:
uuid:6b92ed10-e35a-41f7-ac59-32617f43caed
Source identifiers:
58644
Local pid:
pubs:58644

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