- Abstract:
-
Comprehensive antigenic characterization of a T cell population of unknown specificity is challenging. Existing MHC class I expression systems are limited by the practical difficulty of probing cell populations with an MHC class I peptide library and the cross-reactivity of T cells that are able to recognise many variants of an index peptide. Using emulsion PCR and emulsion in vitro transcription/translation of a random library of peptides conjugated to CD8-null HLA-A*0201 on beads, we probed...
Expand abstract - Publication status:
- Published
- Publisher copy:
- 10.1016/j.jim.2013.11.023
-
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- Journal:
- Journal of immunological methods
- Volume:
- 403
- Issue:
- 1-2
- Pages:
- 72-78
- Publication date:
- 2014-01-05
- DOI:
- EISSN:
-
1872-7905
- ISSN:
-
0022-1759
- URN:
-
uuid:6b8b86c6-b96a-43bc-9e1b-efdaa6eaea88
- Source identifiers:
-
441671
- Local pid:
- pubs:441671
- Language:
- English
- Keywords:
- Copyright date:
- 2014
Journal article
Combinatorial HLA-peptide bead libraries for high throughput identification of CD8⁺ T cell specificity.
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