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Journal article

Combinatorial HLA-peptide bead libraries for high throughput identification of CD8⁺ T cell specificity.

Abstract:

Comprehensive antigenic characterization of a T cell population of unknown specificity is challenging. Existing MHC class I expression systems are limited by the practical difficulty of probing cell populations with an MHC class I peptide library and the cross-reactivity of T cells that are able to recognise many variants of an index peptide. Using emulsion PCR and emulsion in vitro transcription/translation of a random library of peptides conjugated to CD8-null HLA-A*0201 on beads, we probed...

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Publication status:
Published

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Publisher copy:
10.1016/j.jim.2013.11.023

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine, Biomedical Research Centre
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Journal:
Journal of immunological methods
Volume:
403
Issue:
1-2
Pages:
72-78
Publication date:
2014-01-05
DOI:
EISSN:
1872-7905
ISSN:
0022-1759
URN:
uuid:6b8b86c6-b96a-43bc-9e1b-efdaa6eaea88
Source identifiers:
441671
Local pid:
pubs:441671

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