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A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching.

Abstract:

Photoswitchable fluorescent proteins have enabled new approaches for imaging cells, but their utility has been limited either because they cannot be switched repeatedly or because the wavelengths for switching and fluorescence imaging are strictly coupled. We report a bright, monomeric, reversibly photoswitchable variant of GFP, Dreiklang, whose fluorescence excitation spectrum is decoupled from that for optical switching. Reversible on-and-off switching in living cells is accomplished at ill...

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Publication status:
Published

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Publisher copy:
10.1038/nbt.1952

Authors


Brakemann, T More by this author
Andresen, M More by this author
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Journal:
Nature biotechnology
Volume:
29
Issue:
10
Pages:
942-947
Publication date:
2011-10-05
DOI:
EISSN:
1546-1696
ISSN:
1087-0156
URN:
uuid:6b8b22a4-fd26-49a2-8917-a6091bd20503
Source identifiers:
222205
Local pid:
pubs:222205

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