Journal article icon

Journal article

A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching.

Abstract:

Photoswitchable fluorescent proteins have enabled new approaches for imaging cells, but their utility has been limited either because they cannot be switched repeatedly or because the wavelengths for switching and fluorescence imaging are strictly coupled. We report a bright, monomeric, reversibly photoswitchable variant of GFP, Dreiklang, whose fluorescence excitation spectrum is decoupled from that for optical switching. Reversible on-and-off switching in living cells is accomplished at ill...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1038/nbt.1952

Authors


Expand authors...
Journal:
Nature biotechnology
Volume:
29
Issue:
10
Pages:
942-947
Publication date:
2011-10-01
DOI:
EISSN:
1546-1696
ISSN:
1087-0156
Source identifiers:
222205
Language:
English
Keywords:
Pubs id:
pubs:222205
UUID:
uuid:6b8b22a4-fd26-49a2-8917-a6091bd20503
Local pid:
pubs:222205
Deposit date:
2012-12-19

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP