Journal article icon

Journal article

A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transfer.

Abstract:

Bioluminescence resonance energy transfer (BRET), which relies on nonradiative energy transfer between luciferase-coupled donors and GFP-coupled acceptors, is emerging as a useful tool for analyzing the quaternary structures of cell-surface molecules. Conventional BRET analyses are generally done at maximal expression levels and single acceptor/donor ratios. We show that under these conditions substantial energy transfer arises from random interactions within the membrane. The dependence of B...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1038/nmeth978

Authors


Oliveira, MI More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, RDM, Molecular Medicine
Journal:
Nature methods
Volume:
3
Issue:
12
Pages:
1001-1006
Publication date:
2006-12-05
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
URN:
uuid:6b853319-0d62-4735-9b20-a7f2eea1bf2d
Source identifiers:
15862
Local pid:
pubs:15862

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP