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siRNA modified with 2′‐deoxy‐2′‐C‐methylpyrimidine nucleosides

Abstract:

(2′S)‐2′‐Deoxy‐2′‐C‐methyluridine and (2′R)‐2′‐deoxy‐2′‐C‐methyluridine were incorporated in the 3′‐overhang region of the sense and antisense strands and in positions 2 and 5 of the seed region of siRNA duplexes directed against Renilla luciferase, whereas (2′S)‐2′‐deoxy‐2′‐C‐methylcytidine was incorporated in the 6‐position of the seed region of the same constructions. A dual luciferase reporter assay in transfected HeLa cells was used as a model system to measure the IC50 values of 24 diff...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1002/cbic.201800077

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
ORCID:
0000-0001-6421-7733
More from this funder
Name:
EMBO short-term fellowship
Funding agency for:
Dellafiore, M
Grant:
ASTF 515–2015
More from this funder
Name:
Universidad Nacional de Quilmes
Grant:
1400/15
More from this funder
Name:
Generalitat de Catalunya
Grant:
2014SGR 187
More from this funder
Name:
Spanish Ministry of Education
Grant:
CTQ2014-52588-R
More from this funder
Name:
Argentinian Ministry of Science and Technology
Grant:
PICT-2008–2194
Publisher:
Wiley
Journal:
ChemBioChem More from this journal
Volume:
19
Issue:
13
Pages:
1409-1413
Publication date:
2018-05-30
Acceptance date:
2018-04-14
DOI:
EISSN:
1439-7633
ISSN:
1439-4227
Pmid:
29656613
Language:
English
Keywords:
Pubs id:
pubs:836610
UUID:
uuid:6b5d10ae-b334-47ed-9aca-eb899844c313
Local pid:
pubs:836610
Source identifiers:
836610
Deposit date:
2018-06-07

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