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siRNA modified with 2′‐deoxy‐2′‐C‐methylpyrimidine nucleosides

Abstract:

(2′S)‐2′‐Deoxy‐2′‐C‐methyluridine and (2′R)‐2′‐deoxy‐2′‐C‐methyluridine were incorporated in the 3′‐overhang region of the sense and antisense strands and in positions 2 and 5 of the seed region of siRNA duplexes directed against Renilla luciferase, whereas (2′S)‐2′‐deoxy‐2′‐C‐methylcytidine was incorporated in the 6‐position of the seed region of the same constructions. A dual luciferase reporter assay in transfected HeLa cells was used as a model system to measure the IC50 values of 24 diff...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1002/cbic.201800077

Authors


Dellafiore, M More by this author
Aviñó, A More by this author
More by this author
Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
Pathology Dunn School
ORCID:
0000-0001-6421-7733
Montserrat, JM More by this author
Iribarren, AM More by this author
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Funding agency for:
Dellafiore, M
Publisher:
Wiley Publisher's website
Journal:
ChemBioChem Journal website
Volume:
19
Issue:
13
Pages:
1409-1413
Publication date:
2018-05-30
Acceptance date:
2018-04-14
DOI:
EISSN:
1439-7633
ISSN:
1439-4227
Pubs id:
pubs:836610
URN:
uri:6b5d10ae-b334-47ed-9aca-eb899844c313
UUID:
uuid:6b5d10ae-b334-47ed-9aca-eb899844c313
Local pid:
pubs:836610

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