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Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing

Abstract:
We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas.
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1038/nmeth.3321

Authors


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Department:
Wolfson College
Mercer, TR More by this author
Bussotti, G More by this author
Publisher:
Nature Research Publisher's website
Journal:
Nature methods Journal website
Volume:
12
Issue:
4
Pages:
339-342
Publication date:
2015-04-21
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Pubs id:
pubs:519302
URN:
uri:6b1bdf65-c9ab-43fa-af5c-89aff151e16f
UUID:
uuid:6b1bdf65-c9ab-43fa-af5c-89aff151e16f
Local pid:
pubs:519302
Language:
English
Keywords:

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