Journal article
Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing
- Abstract:
- We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Accepted manuscript, pdf, 8.4MB)
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- Publisher copy:
- 10.1038/nmeth.3321
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Authors
Bibliographic Details
- Publisher:
- Nature Research Publisher's website
- Journal:
- Nature methods Journal website
- Volume:
- 12
- Issue:
- 4
- Pages:
- 339-342
- Publication date:
- 2015-04-01
- Acceptance date:
- 2015-02-01
- DOI:
- EISSN:
-
1548-7105
- ISSN:
-
1548-7091
- Pmid:
-
25751143
- Source identifiers:
-
519302
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:519302
- UUID:
-
uuid:6b1bdf65-c9ab-43fa-af5c-89aff151e16f
- Local pid:
- pubs:519302
- Deposit date:
- 2017-01-23
Terms of use
- Copyright holder:
- *Name of copyright holder*
- Copyright date:
- 2015
- Notes:
- © 2015 Nature America, Inc. This is the accepted manuscript version of the article. The final version is available online from Nature Research at: 10.1038/nmeth.3321
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