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Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing

Abstract:
We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/nmeth.3321

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Institution:
University of Oxford
Oxford college:
Wolfson College
Role:
Author
Publisher:
Nature Research Publisher's website
Journal:
Nature methods Journal website
Volume:
12
Issue:
4
Pages:
339-342
Publication date:
2015-04-01
Acceptance date:
2015-02-01
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Pmid:
25751143
Source identifiers:
519302
Language:
English
Keywords:
Pubs id:
pubs:519302
UUID:
uuid:6b1bdf65-c9ab-43fa-af5c-89aff151e16f
Local pid:
pubs:519302
Deposit date:
2017-01-23

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