- Abstract:
-
Improved methods for the detection and characterization of carried Neisseria meningitidis isolates are needed. We evaluated a multiplex PCR algorithm for the detection of a variety of carriage strains in the meningitis belt. To further improve the sensitivity and specificity of the existing PCR assays, primers for gel-based PCR assays (sodC, H, Z) and primers/probe for real-time quantitative PCR (qPCR) assays (porA, cnl, sodC, H, E, Z) were modified or created using Primer Express software. O...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Publisher's version
- Files:
-
-
(pdf, 795.1kb)
-
- Publisher copy:
- 10.1371/journal.pone.0206453
-
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- Publisher:
- Public Library of Science Publisher's website
- Journal:
- PLoS ONE Journal website
- Volume:
- 13
- Issue:
- 12
- Pages:
- Article: e0206453
- Publication date:
- 2018-12-05
- Acceptance date:
- 2018-10-12
- DOI:
- ISSN:
-
1932-6203
- Pubs id:
-
pubs:949934
- URN:
-
uri:6b0bf58a-5c88-42bd-b225-f1d627c7cb40
- UUID:
-
uuid:6b0bf58a-5c88-42bd-b225-f1d627c7cb40
- Local pid:
- pubs:949934
- Copyright holder:
- Diallo et al.
- Copyright date:
- 2018
- Notes:
- This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Journal article
Development of a PCR algorithm to detect and characterize Neisseria meningitidis carriage isolates in the African meningitis belt
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