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Development of a PCR algorithm to detect and characterize Neisseria meningitidis carriage isolates in the African meningitis belt

Abstract:

Improved methods for the detection and characterization of carried Neisseria meningitidis isolates are needed. We evaluated a multiplex PCR algorithm for the detection of a variety of carriage strains in the meningitis belt. To further improve the sensitivity and specificity of the existing PCR assays, primers for gel-based PCR assays (sodC, H, Z) and primers/probe for real-time quantitative PCR (qPCR) assays (porA, cnl, sodC, H, E, Z) were modified or created using Primer Express software. O...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

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Publisher copy:
10.1371/journal.pone.0206453

Authors


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Institution:
University of Oxford
Division:
MPLS Division
Department:
Zoology
Coulibaly, MD More by this author
Rebbetts, LS More by this author
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Institution:
University of Oxford
Division:
MPLS Division
Department:
Zoology
Lucidarme, J More by this author
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Publisher:
Public Library of Science Publisher's website
Journal:
PLoS ONE Journal website
Volume:
13
Issue:
12
Pages:
Article: e0206453
Publication date:
2018-12-05
Acceptance date:
2018-10-12
DOI:
ISSN:
1932-6203
Pubs id:
pubs:949934
URN:
uri:6b0bf58a-5c88-42bd-b225-f1d627c7cb40
UUID:
uuid:6b0bf58a-5c88-42bd-b225-f1d627c7cb40
Local pid:
pubs:949934

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